The Peres-Horodecki criteria for a 2*2 state states that if the smallest eigenvalue of the partial transpose of the state is negative, it is entangled, else it is separable.
According to this paper (page 4, left side), the following is an equivalent formulation to express the above criterion.
Assume the matrix in question looks like this:
$$\begin{bmatrix} \rho_{00,00} & \rho_{00,01} & \rho_{00,10} & \rho_{00,11} \\ \rho_{01,00} & \rho_{01,01} & \rho_{01,10} & \rho_{01,11} \\ \rho_{10,00} & \rho_{10,01} & \rho_{10,10} & \rho_{10,11} \\ \rho_{11,00} & \rho_{11,01} & \rho_{11,10} & \rho_{11,11} \\ \end{bmatrix}$$
Consider the following three determinants:
$$ W_2 = \begin{vmatrix} \rho_{00,00} & \rho_{01,00} \\ \rho_{00,01} & \rho_{01,01} \\ \end{vmatrix} $$
$$W_3 = \begin{vmatrix} \rho_{00,00} & \rho_{01,00} & \rho_{00,10} \\ \rho_{00,01} & \rho_{01,01} & \rho_{00,11} \\ \rho_{10,00} & \rho_{11,00} & \rho_{10,10} \\ \end{vmatrix}$$
$$W_4 = \begin{vmatrix} \rho_{00,00} & \rho_{01,00} & \rho_{00,10} & \rho_{01,10} \\ \rho_{00,01} & \rho_{01,01} & \rho_{00,11} & \rho_{01,11}\\ \rho_{10,00} & \rho_{11,00} & \rho_{10,10} & \rho_{11,10}\\ \rho_{10,01} & \rho_{11,01} & \rho_{10,11} & \rho_{11,11}\\ \end{vmatrix}$$
Notice that $W_4$ is the determinant of the partial transpose of the matrix and $W_3$ and $W_2$ are the first 3*3 and 2*2 elements of the partial transpose.
The condition is if $W_2 \geq 0$ and ($W_3 < 0$ or $W_4 < 0$), then the state is entangled. If not, it is separable.
How are these two equivalent?
Also, can this method be extended to ensure the smallest eigenvalue is greater than any $x$, where $x$ is not necessarily 0?